High throughput and high content screening

Overview

The TDDP will operate on a ‘user-assisted’ basis, where the researcher will perform their screen or experiment with the assistance of the TDDP staff. The TDDP will also offer the following services to researchers:

  • Consultation about screen design and assay workflows
  • Designing custom screening solutions and packages
  • Assistance with grant applications (relating to screening aims)
  • Enabling access to biological/chemical libraries for screening
  • Equipment training and inductions
  • General screening consumables and reagents
  • Boutique applications and pilot experiments using automation

Click here to see a video of the TDDP robotics in action

Equipment

Compound and High Throughput (HTS) Workstation

Housed in a BSCII cabinet, the HTS workstation contains the following instruments/equipment:

  • 6-axis Robotic Arm and dynamic scheduling control software
  • JANUS Automated Liquid Handling Workstation (equipped with pin tool/tip head)
  • EnVision Multi-Mode Plate Reader (Alphascreen enabled)
  • Lid Handler & Barcode Reader
  • Liconic Microplate 37oC CO2 incubator
  • Plate stacker
  • Plate Heat Sealer and Peeler
  • BioTek ELx405 Plate Washer
  • MultiDrop Reagent Dispenser
  • Automated Plate Peeler

High Content Screening and Imaging (HCS) Workstation

Also housed in a BSCII cabinet, the HCS workstation contains the following instruments/equipment:

  • 6-axis Robotic Arm and dynamic scheduling control software
  • JANUS Mini Automated Liquid Handling Workstation (equipped with pin tool/tip head)
  • Opera Phenix high content imaging system
  • Liconic Microplate 37oC CO2 incubator
  • Automated BioTek ELx406 Plate Washer and Reagent Dispenser
  • Lid Handler & Barcode Reader
  • Plate stacker
  • High throughput FACS analyser* (funding permitting)

Other equipment

The TDDP will have a dedicated BSCII hood and 37oC CO2 incubator for cell culture, a cell counter, and centrifuge enabled for microplate spins. A computer with image analysis software (booking required) will also be available.

Libraries

The screening library requirements of researchers are quite diverse; therefore it is not cost effective to order in and stock many different libraries. Instead, the TDDP will purchase a limited number of libraries to be stored in-house, and consult with individual users about their specific requirements. If a library is not stored within the TDDP, access to libraries that are available for researchers to access at academic rates will be enabled.

The types of drug/compound libraries that are available include, but are not limited to the “Australian Chemome” – encompassing Naturebank and other nature product collections, FDA-approved compound libraries, epigenetic modifying agents etc. For screeners that are interested in performing loss of function screens (e.g. RNAi, CRISPR), we have purchased the Dharmacon siGENOME SMARTpool human siRNA library (>18,000 protein coding targets), and we plan to purchase a CRISPR library in the near future (funding permitting). The TDDP can also enable the purchase of boutique RNAi/CRISPR/drug libraries from commercial sources depending on the specific requirement of the user.

Price

Please contact the BRF to enquire about the price.

Phone: 612 54326

Email: tddp.brf@anu.edu.au

Updated:  23 September 2017/Responsible Officer:  Director, ACRF/Page Contact:  Web Admin, ACRF