How to guides

Opening a LIMS account

What is dnaLIMS? LIMS stands for Laboratory Information Management System.  At our facility we use LIMS to: accept orders for, and process, Sanger sequencing work accept orders for Peptide synthesis work sell a range of...

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Order forms

Here are order forms for all services except those accessed via dnaLIMS. If you need more information about a service before placing your order, please go to the relevant section under Our Services. You will be able to access the...

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Preparing a good template for sanger sequencing

The quality of the DNA template is one of the most critical factors in Sanger sequencing.  Please ensure that the template is at the correct concentration, that it is free of contaminants and dissolved in distilled water (...

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Sanger Sequencing - core prep samples

The BRF will perform the reactions for you if you elect to use the "Core Prep" service. Please provide templates and primers at the required concentration and volume in 200µL tubes.  Dilute DNA and primers in water. Recommended...

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Sanger sequencing - user prep samples

The BRF uses an AB 3730 capillary sequencer.  We recommend the protocols set out below.  Please ensure the follow the appropriate precipitation protocol for either 96-well reaction plates OR microfuge tubes. Big Dye terminator 1...

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Sanger sequencing hints, troubleshooting & software

Trouble shooting We recommend these articles from around the world as useful starting points for unexpected problems. http://www.nucleics.com/DNA_sequencing_support/DNA-sequencing-troubleshooting.html DNA Sequencing Software A...

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Updated:  28 July 2017/Responsible Officer:  Director, ACRF/Page Contact:  Web Admin, ACRF